TY  - BOOK
AU  - Julio E. Celis
AU  - Celis,J.E.
TI  - Cell Biology: a laboratory handbook 
SN  - 0121647307
AV  - QH583.2 .C45 2006
U1  - 571.6072 22
PY  - 2006///]
CY  - Amsterdam, Boston
PB  - Elsevier Academic
KW  - Cytology
KW  - Laboratory manuals
KW  - Cytological Techniques
KW  - Culture Techniques
KW  - Laboratory Manuals
KW  - fast
N1  - Includes bibliographical references and index; Volume 1 -- -- Part A. Cell and Tissue Culture : Associated Techniques -- General techniques -- Culture of specific cell types : Stem cells -- Culture of specific cell types : Haemopoietic, mesenchymal, and epithelial -- Differentiation and reprogramming of somatic cells -- Immortalisation -- Somatic cell hybrids -- Cell separation techniques -- Cell cycle analysis -- Cytoxic and cell growth assays -- Apoptosis -- Assays of cell transformation : Tumorigenesis, invasion and wound healing -- Electrophysiological methods -- Organ cultures -- Part B. Virusus -- Growth and purification of viruses -- Part C. Antibodies -- Production and purification of antibodies -- Part D. Immunocytochemistry -- Immunofluoresence -- Part E. Appendix -- -- Volume 2 -- -- Part A. Organelles and Cellular Structures -- Isolation : Plasma, membrane, organelles, and cellular structures -- Vital staining of cells/organelles -- Protein purification -- Part B. Assays -- Endocytic and exocytic pathways -- Membranes -- Mitochondria -- Nuclear transport -- Chromatin assembly -- Signal transduction assays -- Assays and models of in vitro and in vitro motility -- Mechanical stress in single cells -- Part C. Appendix; Volume 3 -- -- Part A. Imaging Techniques -- Light microscopy -- Digital video microscopy -- Confocal microscopy of living cells and fixed cells -- Fluorescent microscopy of living cells -- Use of fluorescent dyes for studies of intracellular physiological parameters -- Digital image processing, analysis, storage, and display -- Part B. Electron Microscopy -- Specimen preparation techniques -- Cryotechniques -- Electron microscopy studies of the cytoskeleton -- Immunoelectron microscopy -- Part C. Scanning Probe and Scanning Electron Microscopy -- Scanning probe and scanning electron microscopy -- Part D. Microdissection -- Tissue and chromosome microdissection -- Part E. Tissue Arrays -- Tissue arrays -- Part F. Cytogenetics and In Situ Hybridisation -- Cytogenetics -- In situ hybridisation -- Part G. Genomics -- Genomics -- Part H. Transgenic, Knockouts, and Knockdown Methods -- Transgenic, knockouts, and knockdown methods -- -- Volume 4 -- -- Part A. Transfer of Macromolecules -- Proteins -- Genes -- Somatic cel nuclear transfer -- Part B. Expression Systems -- Espression systems -- Part C. Gene Expression Profiling -- Differential gene expression -- Part D. Proteins -- Protein determination and analysis -- Sample fractionation for proteomics -- Gel electrophoresis -- Detection of proteins in gels -- Gel profiling of posttranslationally modified proteins -- Protein/protein and protein/small molecule interactions -- Functional proteomics -- Protein/DNA interactions -- Protein degradation -- Mass spectrometry : Protein identification and interactions -- Part E. Appendix
N2  - This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: * Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) * Organelle and Cellular Structures, Assays (Volume 2) * Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) * Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4). -- Publisher description
ER  -